"It's an ankle injury. We're not sure how bad," coach Vance Joseph said. "We're kind of assuming it's not that bad."
Wolfe, 27, was injured when he became tangled up with offensive tackle Menelik Watson in a team drill. Trainers helped Wolfe onto a cart and he was taken to the locker room. He also was sent for an MRI exam following practice.
Wolfe, like most starters, played very little in the Broncos' 24-17 victory at Soldier Field on Thursday night.
Wolfe recorded 51 tackles and 5.5 sacks in 14 games last season. Since being drafted in the second round in 2012, he owns 191 tackles and 22.5 sacks in 69 games (all starts).